Vitamin c regulates cftr???

[dramamama]

Never in all of my time researching have I ever seen this article..I have no idea how I missed it.

1: Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3691-6. Epub 2004 Mar 1. Links
Vitamin C controls the cystic fibrosis transmembrane conductance regulator chloride channel.

Fischer H,
Schwarzer C,
Illek B.
Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA.
Vitamin C (l-ascorbate) is present in the respiratory lining fluid of human lungs, and local deficits occur during oxidative stress. Here we report a unique function of vitamin C on the cystic fibrosis (CF) transmembrane conductance regulator (CFTR), a cAMP-dependent Cl channel that regulates epithelial surface fluid secretion. Vitamin C (100 microM) induced the openings of CFTR Cl channels by increasing its average open probability from 0 to 0.21 +/- 0.08, without a detectable increase in intracellular cAMP levels. Exposure of the apical airway surface to vitamin C stimulated the transepithelial Cl secretion to 68% of forskolin-stimulated currents. The average half-maximal stimulatory constant was 36.5 +/- 2.9 microM, which corresponds to physiological concentrations. When vitamin C was instilled into the nasal epithelium of human subjects, it effectively activated Cl transport in vivo. In CF epithelia, previous treatment of the underlying trafficking defect with trimethylamine oxide or expression of WT CFTR restored the activation of Cl transport by vitamin C. Sodium dependency and phloretin sensitivity, as well as the expression of transcripts for sodium-dependent vitamin C transporter (SVCT)-1 and SVCT2, support a model in which an apical vitamin C transporter is central for relaying the effect of vitamin C to CFTR. We conclude that cellular vitamin C is a biological regulator of CFTR-mediated Cl secretion in epithelia. The pool of vitamin C in the respiratory tract represents a potential nutraceutical and pharmaceutical target for the complementary treatment of sticky airway secretions by enhancing epithelial fluid secretion.
PMID: 14993613 [PubMed - indexed f

The whole article can be found here.
<a target=_blank class=ftalternatingbarlinklarge href="http://www.pnas.org/cgi/reprint/101/10/3691.pdf
">http://www.pnas.org/cgi/reprint/101/10/3691.pdf
</a>

<b>Ok, so when I had my vit c level tested, it was almost undetectable a few months ago.... I wonder if adding a little extra vitamin c wold help?</b>

 

[dramamama]

Never in all of my time researching have I ever seen this article..I have no idea how I missed it.

1: Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3691-6. Epub 2004 Mar 1. Links
Vitamin C controls the cystic fibrosis transmembrane conductance regulator chloride channel.

Fischer H,
Schwarzer C,
Illek B.
Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA.
Vitamin C (l-ascorbate) is present in the respiratory lining fluid of human lungs, and local deficits occur during oxidative stress. Here we report a unique function of vitamin C on the cystic fibrosis (CF) transmembrane conductance regulator (CFTR), a cAMP-dependent Cl channel that regulates epithelial surface fluid secretion. Vitamin C (100 microM) induced the openings of CFTR Cl channels by increasing its average open probability from 0 to 0.21 +/- 0.08, without a detectable increase in intracellular cAMP levels. Exposure of the apical airway surface to vitamin C stimulated the transepithelial Cl secretion to 68% of forskolin-stimulated currents. The average half-maximal stimulatory constant was 36.5 +/- 2.9 microM, which corresponds to physiological concentrations. When vitamin C was instilled into the nasal epithelium of human subjects, it effectively activated Cl transport in vivo. In CF epithelia, previous treatment of the underlying trafficking defect with trimethylamine oxide or expression of WT CFTR restored the activation of Cl transport by vitamin C. Sodium dependency and phloretin sensitivity, as well as the expression of transcripts for sodium-dependent vitamin C transporter (SVCT)-1 and SVCT2, support a model in which an apical vitamin C transporter is central for relaying the effect of vitamin C to CFTR. We conclude that cellular vitamin C is a biological regulator of CFTR-mediated Cl secretion in epithelia. The pool of vitamin C in the respiratory tract represents a potential nutraceutical and pharmaceutical target for the complementary treatment of sticky airway secretions by enhancing epithelial fluid secretion.
PMID: 14993613 [PubMed - indexed f

The whole article can be found here.
<a target=_blank class=ftalternatingbarlinklarge href="http://www.pnas.org/cgi/reprint/101/10/3691.pdf
">http://www.pnas.org/cgi/reprint/101/10/3691.pdf
</a>

<b>Ok, so when I had my vit c level tested, it was almost undetectable a few months ago.... I wonder if adding a little extra vitamin c wold help?</b>

 

[dramamama]

Never in all of my time researching have I ever seen this article..I have no idea how I missed it.

1: Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3691-6. Epub 2004 Mar 1. Links
Vitamin C controls the cystic fibrosis transmembrane conductance regulator chloride channel.

Fischer H,
Schwarzer C,
Illek B.
Children's Hospital Oakland Research Institute, 5700 Martin Luther King Jr. Way, Oakland, CA 94609, USA.
Vitamin C (l-ascorbate) is present in the respiratory lining fluid of human lungs, and local deficits occur during oxidative stress. Here we report a unique function of vitamin C on the cystic fibrosis (CF) transmembrane conductance regulator (CFTR), a cAMP-dependent Cl channel that regulates epithelial surface fluid secretion. Vitamin C (100 microM) induced the openings of CFTR Cl channels by increasing its average open probability from 0 to 0.21 +/- 0.08, without a detectable increase in intracellular cAMP levels. Exposure of the apical airway surface to vitamin C stimulated the transepithelial Cl secretion to 68% of forskolin-stimulated currents. The average half-maximal stimulatory constant was 36.5 +/- 2.9 microM, which corresponds to physiological concentrations. When vitamin C was instilled into the nasal epithelium of human subjects, it effectively activated Cl transport in vivo. In CF epithelia, previous treatment of the underlying trafficking defect with trimethylamine oxide or expression of WT CFTR restored the activation of Cl transport by vitamin C. Sodium dependency and phloretin sensitivity, as well as the expression of transcripts for sodium-dependent vitamin C transporter (SVCT)-1 and SVCT2, support a model in which an apical vitamin C transporter is central for relaying the effect of vitamin C to CFTR. We conclude that cellular vitamin C is a biological regulator of CFTR-mediated Cl secretion in epithelia. The pool of vitamin C in the respiratory tract represents a potential nutraceutical and pharmaceutical target for the complementary treatment of sticky airway secretions by enhancing epithelial fluid secretion.
PMID: 14993613 [PubMed - indexed f

The whole article can be found here.
<a target=_blank class=ftalternatingbarlinklarge href="http://www.pnas.org/cgi/reprint/101/10/3691.pdf
">http://www.pnas.org/cgi/reprint/101/10/3691.pdf
</a>

<b>Ok, so when I had my vit c level tested, it was almost undetectable a few months ago.... I wonder if adding a little extra vitamin c wold help?</b>

 

[dramamama]

oh no!!! I have to up my vit c!!!

Plasma vitamin C concentrations in patients with cystic fibrosis: evidence of associations with lung inflammation.

Winklhofer-Roob BM,
Ellemunter H,
Fruhwirth M,
Schlegel-Haueter SE,
Khoschsorur G,
van't Hof MA,
Shmerling DH.
Department of Pediatrics, University of Zurich, Switzerland.
Vitamin C status and possible associations with the disease process in cystic fibrosis (CF) patients were investigated. Plasma vitamin C concentrations in patients from two different mid-European populations (Swiss, n = 62; Austrian, n = 60) taking no or low-dose vitamin C from multivitamin supplements did not differ from each other or from control subjects (n = 34). Vitamin C concentrations decreased with age (5.05 mumol.L-1, y-1). When followed up for 12 mo, patients had the highest plasma vitamin C concentrations in February and the lowest in May and August (P < 0.01); the decrease in vitamin C was accompanied by increases in plasma malondialdehyde (P < 0.001) and tumor necrosis factor alpha concentrations (P < 0.01). During supplementation with vitamin E for 2 mo or beta-carotene for 12 mo vitamin C concentrations did not change. They correlated inversely with white blood cell count (r = -0.36, P = 0.008), bands (r = -0.36, P = 0.02), alpha 1-acid glycoprotein (r = -0.45, P = 0.002), interleukin 6 (r = -0.46, P = 0.0006), and neutrophil elastase/alpha 1-proteinase inhibitor complexes (r = -0.34, P = 0.02). <b>In patients with vitamin C concentrations < 40 mumol/L, all indexes of inflammation were relatively high, whereas those with concentrations > 80 mumol/L (upper quartile of control subjects) showed clearly lower values. These results are consistent with the hypothesis that by scavenging oxygen free radicals vitamin C interacts with an inflammation-amplifying cycle of activation of alveolar macrophages and neutrophils, release of proinflammatory cytokines and oxygen free radicals, and inactivation of antiproteases.
</b>PMID: 9174484 [PubMed - indexed for MEDLINE]

 

[dramamama]

oh no!!! I have to up my vit c!!!

Plasma vitamin C concentrations in patients with cystic fibrosis: evidence of associations with lung inflammation.

Winklhofer-Roob BM,
Ellemunter H,
Fruhwirth M,
Schlegel-Haueter SE,
Khoschsorur G,
van't Hof MA,
Shmerling DH.
Department of Pediatrics, University of Zurich, Switzerland.
Vitamin C status and possible associations with the disease process in cystic fibrosis (CF) patients were investigated. Plasma vitamin C concentrations in patients from two different mid-European populations (Swiss, n = 62; Austrian, n = 60) taking no or low-dose vitamin C from multivitamin supplements did not differ from each other or from control subjects (n = 34). Vitamin C concentrations decreased with age (5.05 mumol.L-1, y-1). When followed up for 12 mo, patients had the highest plasma vitamin C concentrations in February and the lowest in May and August (P < 0.01); the decrease in vitamin C was accompanied by increases in plasma malondialdehyde (P < 0.001) and tumor necrosis factor alpha concentrations (P < 0.01). During supplementation with vitamin E for 2 mo or beta-carotene for 12 mo vitamin C concentrations did not change. They correlated inversely with white blood cell count (r = -0.36, P = 0.008), bands (r = -0.36, P = 0.02), alpha 1-acid glycoprotein (r = -0.45, P = 0.002), interleukin 6 (r = -0.46, P = 0.0006), and neutrophil elastase/alpha 1-proteinase inhibitor complexes (r = -0.34, P = 0.02). <b>In patients with vitamin C concentrations < 40 mumol/L, all indexes of inflammation were relatively high, whereas those with concentrations > 80 mumol/L (upper quartile of control subjects) showed clearly lower values. These results are consistent with the hypothesis that by scavenging oxygen free radicals vitamin C interacts with an inflammation-amplifying cycle of activation of alveolar macrophages and neutrophils, release of proinflammatory cytokines and oxygen free radicals, and inactivation of antiproteases.
</b>PMID: 9174484 [PubMed - indexed for MEDLINE]

 

[dramamama]

oh no!!! I have to up my vit c!!!

Plasma vitamin C concentrations in patients with cystic fibrosis: evidence of associations with lung inflammation.

Winklhofer-Roob BM,
Ellemunter H,
Fruhwirth M,
Schlegel-Haueter SE,
Khoschsorur G,
van't Hof MA,
Shmerling DH.
Department of Pediatrics, University of Zurich, Switzerland.
Vitamin C status and possible associations with the disease process in cystic fibrosis (CF) patients were investigated. Plasma vitamin C concentrations in patients from two different mid-European populations (Swiss, n = 62; Austrian, n = 60) taking no or low-dose vitamin C from multivitamin supplements did not differ from each other or from control subjects (n = 34). Vitamin C concentrations decreased with age (5.05 mumol.L-1, y-1). When followed up for 12 mo, patients had the highest plasma vitamin C concentrations in February and the lowest in May and August (P < 0.01); the decrease in vitamin C was accompanied by increases in plasma malondialdehyde (P < 0.001) and tumor necrosis factor alpha concentrations (P < 0.01). During supplementation with vitamin E for 2 mo or beta-carotene for 12 mo vitamin C concentrations did not change. They correlated inversely with white blood cell count (r = -0.36, P = 0.008), bands (r = -0.36, P = 0.02), alpha 1-acid glycoprotein (r = -0.45, P = 0.002), interleukin 6 (r = -0.46, P = 0.0006), and neutrophil elastase/alpha 1-proteinase inhibitor complexes (r = -0.34, P = 0.02). <b>In patients with vitamin C concentrations < 40 mumol/L, all indexes of inflammation were relatively high, whereas those with concentrations > 80 mumol/L (upper quartile of control subjects) showed clearly lower values. These results are consistent with the hypothesis that by scavenging oxygen free radicals vitamin C interacts with an inflammation-amplifying cycle of activation of alveolar macrophages and neutrophils, release of proinflammatory cytokines and oxygen free radicals, and inactivation of antiproteases.
</b>PMID: 9174484 [PubMed - indexed for MEDLINE]

 

[greatbay]

I have been on 1000mg of vitamin C for 3 years and when I went to CF Clinic in March after being diag. w/cf in Feb., the doctor asked if I had noticed any difference with the vit. c and I told him that I have NOT had a cold since I started taking it....I have had infections but NO cold like symtoms.....He said, "For God's sake, stay on it!" It has worked for me but may not for others.

 

[greatbay]

I have been on 1000mg of vitamin C for 3 years and when I went to CF Clinic in March after being diag. w/cf in Feb., the doctor asked if I had noticed any difference with the vit. c and I told him that I have NOT had a cold since I started taking it....I have had infections but NO cold like symtoms.....He said, "For God's sake, stay on it!" It has worked for me but may not for others.

 

[greatbay]

I have been on 1000mg of vitamin C for 3 years and when I went to CF Clinic in March after being diag. w/cf in Feb., the doctor asked if I had noticed any difference with the vit. c and I told him that I have NOT had a cold since I started taking it....I have had infections but NO cold like symtoms.....He said, "For God's sake, stay on it!" It has worked for me but may not for others.

 

[amber682]

That's so crazy, I just read something about that last night. I had never heard of it either, but the article mentioned salicylic acid treatment for CF. That's vitamin C right?

This is the website I was on, it's incredibly long and I was just sort of scanning through looking for info on a certain mutation. Somewhere in there is the vit. C thing.

I tried to give you a link but I can't get it to work. I was on www.ncbi.nlm.nih.gov, and I typed Cystic Fibrosis into the search bar, I read #1. If anyone is ever bored and wants to research CF a little, there's a ton of info on this site.

 

[amber682]

That's so crazy, I just read something about that last night. I had never heard of it either, but the article mentioned salicylic acid treatment for CF. That's vitamin C right?

This is the website I was on, it's incredibly long and I was just sort of scanning through looking for info on a certain mutation. Somewhere in there is the vit. C thing.

I tried to give you a link but I can't get it to work. I was on www.ncbi.nlm.nih.gov, and I typed Cystic Fibrosis into the search bar, I read #1. If anyone is ever bored and wants to research CF a little, there's a ton of info on this site.

 

[amber682]

That's so crazy, I just read something about that last night. I had never heard of it either, but the article mentioned salicylic acid treatment for CF. That's vitamin C right?

This is the website I was on, it's incredibly long and I was just sort of scanning through looking for info on a certain mutation. Somewhere in there is the vit. C thing.

I tried to give you a link but I can't get it to work. I was on www.ncbi.nlm.nih.gov, and I typed Cystic Fibrosis into the search bar, I read #1. If anyone is ever bored and wants to research CF a little, there's a ton of info on this site.

 

[mom2lillian]

No, salicylic acid is not vitamin C, it is aspirin. Aspirin has also been noted to reduce inflamation many times over but it can have side effects such as increased bleeding which can be a problem in CF. i tried it for a while but had issues with it and I have problems with hemoptysis so it wasnt for me.

Please post that link here if you can find it.

Thanks!

 

[mom2lillian]

No, salicylic acid is not vitamin C, it is aspirin. Aspirin has also been noted to reduce inflamation many times over but it can have side effects such as increased bleeding which can be a problem in CF. i tried it for a while but had issues with it and I have problems with hemoptysis so it wasnt for me.

Please post that link here if you can find it.

Thanks!

 

[mom2lillian]

No, salicylic acid is not vitamin C, it is aspirin. Aspirin has also been noted to reduce inflamation many times over but it can have side effects such as increased bleeding which can be a problem in CF. i tried it for a while but had issues with it and I have problems with hemoptysis so it wasnt for me.

Please post that link here if you can find it.

Thanks!